Alkylresorcinols in cereal grains

Alkylresorcinols are phenolic lipids present at levels of up to 0.15% of whole grain wheat and rye, but little is known about their presence in food, absorption in animals and humans, and their in vivo biological effects. Because alkylresorcinols are present in the human diet in significant amounts only in products containing whole grain wheat or rye, they have potential to be biomarkers of whole grain wheat and rye intake. This thesis describes some of the research undertaken to investigate whether alkylresorcinols could be biomarkers of whole grain wheat and rye intake. A rapid gas chromatographic method was developed to analyse alkylresorcinols in whole cereal grains. This method was then applied to detect the presence and amount of alkylresorcinols in several cereal grains. Wheat, rye and triticale all contain moderate to high amounts of alkylresorcinols (300-1500 µg/g), while barley contains low amounts (~50 µg/g). In these cereals, alkylresorcinols are present in the bran fraction. All other cereals analysed (rice, oats, maize, sorghum and millet) did not contain any detectable amounts of alkylresorcinols. Previous studies have suggested that alkylresorcinols are destroyed by the baking process. However, an extraction method using hot propanol:water was able to recover all alkylresorcinols from experimental breads, indicating that alkylresorcinols are not destroyed during baking. The absorption of alkylresorcinols in rats, pigs and humans was determined, with values for absorption ranging from 34–79%, depending on the model and the amount of alkylresorcinols consumed. Alkylresorcinols in the plasma of pigs fed a single meal of rye, peaked at 3-4 hours, and remained elevated compared to the baseline levels after 16 hours. Preliminary studies to find alkylresorcinol metabolites in humans suggest that they have their alkyl chains shortened by β-oxidation. The effect of purified rye alkylresorcinols on lipid parameters (tocopherols, cholesterol and fatty acids) was tested on a rat model. Alkylresorcinols did not appear to affect rat performance, but in high amounts they could decrease liver cholesterol, and moderately elevate γ-tocopherol levels. Overall, the results suggest that alkylresorcinols do not have a large effect on lipid absorption/metabolism in rats

Projected speciality career choices amongst undergraduate dental students in Scotland

Background: Despite increased numbers of dental specialists, around 90% of dentists in the UK continue to work in general practice. Previous research shows that undergraduate students indicate interest in speciality careers, however few studies have explored which specialties are of interest, when and why. The aim of this study was to explore whether Bachelor of Dental Surgery (BDS) undergraduates attending Glasgow Dental School (GDS) indicate a desire to pursue a speciality career, why, and the extent to which they feel prepared in this regard. Method: We conducted an internet-based survey (response rate 81%; n = 331) of all GDS students between October and November 2016. Results: One hundred and eighty-six students (56%) had decided to specialise. Seventy-eight percent of these students cited enjoyment of that service, or types of patient seen, as the single most influencing factor on their choice. Oral surgery was the most popular choice where one was indicated (35%), followed by orthodontics (26%). Just 14% of BDS4-5 students felt sufficiently exposed to cases in their chosen speciality. A large majority (88%) said they would like information regarding speciality pathways at BDS3 or before. Conclusions: Educators should provide undergraduate students with information about specialties in a structured way, so that they can consider available options

Whole grains beyond fibre: what can metabolomics tell us about mechanisms?

Dietary fibre alone does not fully explain the frequent association between greater intake of whole grains and reduced risk of disease in observational studies, and other phytochemicals or food structure may also play an important role. For all the observational evidence for the benefits of a whole-grain-rich diet, we have only limited knowledge of the mechanisms behind this reduction in disease risk, aside from the action of specific cereal fibres on reduction of blood cholesterol and the post-prandial glucose peak. Nutritional metabolomics, the global measurement and interpretation of metabolic profiles, assesses the interaction of food with the endogenous gene-protein cascade and the gut microbiome. This approach allows the generation of new hypotheses which account for systemic effects, rather than just focusing on one or two mechanisms or metabolic pathways. To date, animal and human trials using metabolomics to investigate mechanistic changes to metabolism on eating whole grains and cereal fractions have led to new hypotheses around mechanistic effects of whole grains. These include the role of cereals as a major source of dietary glycine betaine, a possible effect on phospholipid synthesis or metabolism, the role of branched-chain amino acids and improvements in insulin sensitivity, and the possibility that whole grains may have an effect on protein metabolism. These hypotheses help explain some of the observed effects of whole grains, although mechanistic studies using stable isotopes and fully quantitative measures are required to confirm these potential mechanisms

Whole grains beyond fibre: what can metabolomics tell us about mechanisms?

Dietary fibre alone does not fully explain the frequent association between greater intake of whole grains and reduced risk of disease in observational studies, and other phytochemicals or food structure may also play an important role. For all the observational evidence for the benefits of a whole-grain-rich diet, we have only limited knowledge of the mechanisms behind this reduction in disease risk, aside from the action of specific cereal fibres on reduction of blood cholesterol and the post-prandial glucose peak. Nutritional metabolomics, the global measurement and interpretation of metabolic profiles, assesses the interaction of food with the endogenous gene-protein cascade and the gut microbiome. This approach allows the generation of new hypotheses which account for systemic effects, rather than just focusing on one or two mechanisms or metabolic pathways. To date, animal and human trials using metabolomics to investigate mechanistic changes to metabolism on eating whole grains and cereal fractions have led to new hypotheses around mechanistic effects of whole grains. These include the role of cereals as a major source of dietary glycine betaine, a possible effect on phospholipid synthesis or metabolism, the role of branched-chain amino acids and improvements in insulin sensitivity, and the possibility that whole grains may have an effect on protein metabolism. These hypotheses help explain some of the observed effects of whole grains, although mechanistic studies using stable isotopes and fully quantitative measures are required to confirm these potential mechanisms

Understanding and responding when things go wrong: key principles for primary care educators

Learning from events with unwanted outcomes is an important part of workplace based education and providing evidence for medical appraisal and revalidation. It has been suggested that adopting a ‘systems approach’ could enhance learning and effective change. We believe the following key principles should be understood by all healthcare staff, especially those with a role in developing and delivering educational content for safety and improvement in primary care. When things go wrong, professional accountability involves accepting there has been a problem, apologising if necessary and committing to learn and change. This is easier in a ‘Just Culture’ where wilful disregard of safe practice is not tolerated but where decisions commensurate with training and experience do not result in blame and punishment. People usually attempt to achieve successful outcomes, but when things go wrong the contribution of hindsight and attribution bias as well as a lack of understanding of conditions and available information (local rationality) can lead to inappropriately blame ‘human error’. System complexity makes reduction into component parts difficult; thus attempting to ‘find-and-fix’ malfunctioning components may not always be a valid approach. Finally, performance variability by staff is often needed to meet demands or cope with resource constraints. We believe understanding these core principles is a necessary precursor to adopting a ‘systems approach’ that can increase learning and reduce the damaging effects on morale when ‘human error’ is blamed. This may result in ‘human error’ becoming the starting point of an investigation and not the endpoint

Perfect Quantum Routing in Regular Spin Networks

Regular families of coupled quantum networks are described such the unknown state of a qubit can be perfectly routed from any node to any other node in a time linear in the distance. Unlike previous constructions, the transfer can be achieved perfectly on a network that is local on any specified number of spatial dimensions. The ability to route the state, and the regularity of the networks, vastly improve the utility of this scheme in comparison to perfect state transfer schemes. The structures can also be used for entanglement generation.Comment: 4 pages, 3 figure

Understanding patient safety performance and educational needs using the ‘Safety-II’ approach for complex systems

Participation in projects to improve patient safety is a key component of general practice (GP) specialty training, appraisal and revalidation. Patient safety training priorities for GPs at all career stages are described in the Royal College of General Practitioners’ curriculum. Current methods that are taught and employed to improve safety often use a ‘find-and-fix’ approach to identify components of a system (including humans) where performance could be improved. However, the complex interactions and inter-dependence between components in healthcare systems mean that cause and effect are not always linked in a predictable manner. The Safety-II approach has been proposed as a new way to understand how safety is achieved in complex systems that may improve quality and safety initiatives and enhance GP and trainee curriculum coverage. Safety-II aims to maximise the number of events with a successful outcome by exploring everyday work. Work-as-done often differs from work-as-imagined in protocols and guidelines and various ways to achieve success, dependent on work conditions, may be possible. Traditional approaches to improve the quality and safety of care often aim to constrain variability but understanding and managing variability may be a more beneficial approach. The application of a Safety-II approach to incident investigation, quality improvement projects, prospective analysis of risk in systems and performance indicators may offer improved insight into system performance leading to more effective change. The way forward may be to combine the Safety-II approach with ‘traditional’ methods to enhance patient safety training, outcomes and curriculum coverage

On vitamin C metabolism in pulmonary tuberculosis

(1) 35 patients suffering from pulmonary tuberculosis were investigated regarding their vitamin C nutrition by urinary excretion and saturation tests. (2) A hypovitaminosis C was found in many, greater in febrile patients with active disease. (3) The average daily excretion test is shown to be unsatisfactory for testing febrile pulmonary tuberculous patients. (4) The results of the workers in this field are reviewed. (5) The possible causes of this hypovitaminosis C are mentioned. (6) The effects of a deficiency or excess of vitamin C are considered, it being concluded that on the whole, if marked, the deficiency will have a deleterious effect. (7) The application of these findings to cases of pulmonary tuberculosis is considered. Febrile patients with active disease should receive 100 mgm.ascorbic acid daily in addition to their ordinary diet. Non-febrile patients should receive plenty of green vegetables and, if possible, fresh fruit

The Application of the Hermeneutic Process to Qualitative Safety Data: A Case Study using Data from the CIRAS project

This article describes the new qualitative methodology developed for use in CIRAS (Confidential Incident Reporting and Analysis System), the confidential database set up for the UK railways by the University of Strathclyde. CIRAS is a project in which qualitative safety data are disidentified and then stored and analysed in a central database. Due to the confidential nature of the data provided, conventional (positivist) methods of checking their accuracy are not applicable; therefore a new methodology was developed - the Applied Hermeneutic Methodology (AHM). Based on Paul Ricoeur's `hermeneutic arc', this methodology uses appropriate computer software to provide a method of analysis that can be shown to be reliable (in the sense that consensus in interpretations between different interpreters can be demonstrated). Moreover, given that the classifiers of the textual elements can be represented in numeric form, AHM crosses the `qualitative-quantitative divide'. It is suggested that this methodology is more rigorous and philosophically coherent than existing methodologies and that it has implications for all areas of the social sciences where qualitative texts are analysed

Information Processing Models: Benefits and Limitations

This paper looks at the three main information processing models from the point of view of researchers in confidential human factors databases. It explores conceptual problems with two of these information processing models, and goes on to explore possible advantages of adopting a ‘connectionist’ paradigm. Links between connectionism and ‘situated cognition’ are demonstrated. Practical work carried out using a connectionist/situated cognition model is described, and the way in which the ‘situatedness’ of discourse can influence the kind of data that can be collected is discussed. Finally it is argued that more emphasis should be placed in ergonomics on sociation, situatedness and embodiment, and that this might help to deal with problems faced in creation and interrogating databases: especially as regards the creation of coherent and reliable ‘coding taxonomies’